Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes

The International Union of Crystallography has published an article by Bjelčić et Al which presents a “methodology employing silicon nitride chips arranged in a `sandwich’ configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples” – there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples.

Silson’s silicon nitride membranes were used for the Anaerobic chip assembly for X-ray crystallography, of membrane size 2.5 × 2.5 mm and thickness 1000 nm, and frame size 5.0 × 5.0 mm, and thickness 200 µm.

Data using the constructed “sandwich” of our membranes was collected at the BioMAX beamline (Macromolecular crystallography) at the MAX IV Laboratory.

Follow this link to read the article in full!


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