Cell Culture on Silicon Nitride Membranes

JoVE Journal published a case study on cell culture on silicon nitride membranes.

Summary: Presented here is a protocol for cell culture on silicon nitride membranes and plunge-freezing prior to X-ray fluorescence imaging with a synchrotron cryogenic X-ray nanoprobe. When only room temperature nano-analysis is provided, the frozen samples can be further freeze-dried. These are critical steps to obtain information on the intracellular elemental composition.

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis.

Case studies

Probing the Local Structure of Na in NaNO3-Promoted, MgO-Based CO2 Sorbents via X-ray Absorption Spectroscopy

Probing the Local Structure of Na in NaNO3-Promoted, MgO-Based CO2 Sorbents via X-ray Absorption Spectroscopy

Chemistry of Materials have published a paper by Rekhtina et Al about studies using "X-ray absorption spectroscopy (...

Intracellular Fate of Sub-Toxic Concentration of Functionalized Selenium Nanoparticles in Aggressive Prostate Cancer Cells

Intracellular Fate of Sub-Toxic Concentration of Functionalized Selenium Nanoparticles in Aggressive Prostate Cancer Cells

Nanomaterials have published an article by Bissardon et Al in which scientists have investigated "the impact of sub-...

Holographic imaging of antiferromagnetic domains with in-situ magnetic field

Holographic imaging of antiferromagnetic domains with in-situ magnetic field

arXiv has published an article by Harrison et Al which investigates the opportunity of extending lensless coherent x...

Proton Microbeam Targeted Irradiation of the Gonad Primordium Region Induces Developmental Alterations Associated with Heat Shock Responses and Cuticle Defense in Caenorhabditis elegans

Proton Microbeam Targeted Irradiation of the Gonad Primordium Region Induces Developmental Alterations Associated with Heat Shock Responses and Cuticle Defense in Caenorhabditis elegans

MDPI have published an article by Beaudier et Al which explains their investigation of "the effects of targeted irra...

Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes

Anaerobic fixed-target serial crystallography using sandwiched silicon nitride membranes

The International Union of Crystallography has published an article by Bjelčić et Al which presents a "methodology e...

Synchrotron-Based X-ray Photoelectron Microscopy of LMO/LAGP/Cu Thin-Film Solid-State Lithium Metal Batteries

Synchrotron-Based X-ray Photoelectron Microscopy of LMO/LAGP/Cu Thin-Film Solid-State Lithium Metal Batteries

MDPI have published an article by Kazemian et Al in their Batteries journal about solid-state batteries (SSB) which ...

Correlating STED and synchrotron XRF nano-imaging to explore metal functions in synapses

Correlating STED and synchrotron XRF nano-imaging to explore metal functions in synapses

AIP Conference Proceedings have published an article by Domart et Al which in which researchers "correlate stimulate...

Employing x-ray spectromicroscopy for the understanding of anisotropy in organic thin film growth

Employing x-ray spectromicroscopy for the understanding of anisotropy in organic thin film growth

AIP Conference Proceedings have published an article by Hawly et Al which reports on the "fabrication of high-qualit...

After the sun: a nanoscale comparison of the surface chemical composition of UV and soil weathered plastics

After the sun: a nanoscale comparison of the surface chemical composition of UV and soil weathered plastics

Microplastics and Nanoplastics has published an article by Foetisch et Al which uses "scanning transmission x-ray mi...