Cell Culture on Silicon Nitride Membranes

JoVE Journal published a case study on cell culture on silicon nitride membranes.

Summary: Presented here is a protocol for cell culture on silicon nitride membranes and plunge-freezing prior to X-ray fluorescence imaging with a synchrotron cryogenic X-ray nanoprobe. When only room temperature nano-analysis is provided, the frozen samples can be further freeze-dried. These are critical steps to obtain information on the intracellular elemental composition.

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis.

Case studies

Soft X-Ray Scanning Transmission Microscopy as a Selective Probe of Topical Dermal Drug Delivery: The Role of Petrolatum and Occlusion

Soft X-Ray Scanning Transmission Microscopy as a Selective Probe of Topical Dermal Drug Delivery: The Role of Petrolatum and Occlusion

The Journal of Electron Spectroscopy and Related Phenomena has published an article by Germer et Al which describes ...

Understanding dose correction for high-resolution 50 kV electron-beam lithography on thick resist layers

Understanding dose correction for high-resolution 50 kV electron-beam lithography on thick resist layers

Micro and Nano Engineering have published an article by Astrand et Al which investigates dose insufficiency when wri...

Superoxide Production under Soft X‑ray Irradiation of Liquid Water

Superoxide Production under Soft X‑ray Irradiation of Liquid Water

In an article published by The Journal of Physical Chemistry, researchers have been investigating Superoxide Product...

10Be at iThemba LABS using a silicon nitride membrane stack as absorber for isobar suppression

10Be at iThemba LABS using a silicon nitride membrane stack as absorber for isobar suppression

Nuclear Inst. and Methods in Physics Research, B have published an article by Winkler et Al about asome ccelerator m...

Stratospheric ozone depletion in the Antarctic region triggers intense changes in sea salt aerosol geochemistry

Stratospheric ozone depletion in the Antarctic region triggers intense changes in sea salt aerosol geochemistry

Nature (Communications Earth & Environment) have published a fascinating article by Gonçalves Jr et Al about str...

Development of General-purpose Dielectric Constant Imaging Unit for SEM and Direct Observation of Samples in Aqueous Solution

Development of General-purpose Dielectric Constant Imaging Unit for SEM and Direct Observation of Samples in Aqueous Solution

Microscopy and Microanalysis have published an article by Ogura et Al about a recently developed "scanning electron-...

Revealing the Role of CO during CO2 Hydrogenation on Cu Surfaces with In Situ Soft X-Ray Spectroscopy

Revealing the Role of CO during CO2 Hydrogenation on Cu Surfaces with In Situ Soft X-Ray Spectroscopy

ACS Publications have published an article by Swallow et Al which investigates "the reactions of H2, CO2, and CO gas...

MXenes and Their Heterostructures with Oxides as Electrodes for Supercapacitors

MXenes and Their Heterostructures with Oxides as Electrodes for Supercapacitors

ProQuest have published a dissertation by K. Prenger about MXenes (two-dimensional (2D) transition metal carbides an...

Remote Ambient Aerosols found in the Southern Great Plains and Eastern North Atlantic

Remote Ambient Aerosols found in the Southern Great Plains and Eastern North Atlantic

ProQuest has published a Thesis by D. Bonanno about an "investigation of various aerosol types and the role they pla...